Enzymology and Protein Chemistry

Enzyme Kinetics

 Kinetics of enzyme is the study of chemical reaction rates which are catalyzed by enzymes the study of an enzyme's kinetics provides inner vision to the catalytic mechanism of the enzyme and its role in metabolism, controlling of its activity in the cell and how does drugs and poisons can inhibit its activity. Enzymes can be manipulated with other molecules called enzyme substrates where target molecules bind to an enzyme's active site. and they are transformed into products by the series of steps called as the enzymatic mechanism of action. Few enzymes attach to multiple substrates and/or results in multiple products. Although these mechanisms are of a complex series of stages, there is Vitaly one rate-determining step which determines the overall kinetics of enzyme. This rate-determining step which may be a chemical reaction or a conformational change involved in the enzyme or substrates, such as those involved in the result of product from the reacted enzyme. Knowledge of the enzyme's structural information is more helpful in visualizing the kinetic data of the process. As the structure can suggest details of substrates and products bind during catalyst processes and about changes occur during the reaction and the role of amino acid residues involved in the mechanism. Some enzymes can change shape significantly while the mechanism goes on; in such type of cases, it is also helpful to determine the enzyme structural information of with and without bound substrate analogues and that which do not undergo the enzymatic reaction.