Enzymology and Protein Chemistry

Clinical Enzymology

Plasma is made up of several functional enzymes, they are effectively secreted into plasma. Such as enzymes involved in blood coagulation process. And in case of other few nonfunctional enzymes in plasma, which are included in coming out from cells of different tissues because of normal wear and tear conditions. Enzymes normal levels in blood are countably very low, but they are drastically high in number during cell death (necrosis) or disease condition. so, assays of these enzymes are very useful in easy diagnosis diseases with accurate results. Enzyme assays are basically depending on the measuring the catalytic activity of the enzyme, but not on the concentration of the enzyme protein. Single enzyme can catalyze many molecules so high sensitivity is involved in measurement and reference ranges for plasma enzymes are determined by assay conditions.