University Stuttgart, Germany
Title: Molecular enzymology of DNA methyltransferases – conformational changes and allosteric regulation
Biography: Albert Jeltsch
DNA methylation is an essential epigenetic chromatin modification. The setup and maintenance of DNA methylation patterns depends on the coordinated activity of DNA methyltransferases (DNMTs) and their allosteric regulation by interacting proteins, other chromatin modifications and post-translational modifications. I will present novel assays for DNMTs including single enzyme assays to study their mechanism and conformationally locked mutants to study allosteric effects. Based on this, recent data regarding the regulation and targeting of DNMTs by allosteric effect will be presented. Moreover, I will present insights into the mechanism of DNMTs regarding target site location, specificity and processivity.