David Rabuka
Catalent Biologics, USA
Title: Developing site-specifically modified ADCs using a chemoenzymatic approach
Biography
Biography: David Rabuka
Abstract
We have developed the SMARTagTM technology platform, which enables precise, programmable, site-selective chemical protein modification. Leveraging the target sequence of formylglycine generating enzyme (FGE), we chemoenzymatically modify proteins to generate a precisely placed aldehyde functionality that can be chemically elaborated. Subsequently, novel ligation chemistry is employed that exploits this “aldehyde tag” site. We will present recent data on our novel protein modification platform and its application to generating novel bioconjugates, including ADCs, utilizing our new conjugation chemistries and linkers. The application of these chemistries to generate site-specifically modified bioconjugates with improved efficacy and safety profiles will be presented. Additionally, we will highlight the progress in developing conjugates with a focus on preclinical studies as well as highlight our progress in cell line development and manufacturing by using this chemoenzymatic approach.