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Preeti Chanalia

Preeti Chanalia

Kurukshetra University, India

Title: Proline iminopeptidase from probiotic L. Plantarum: Physicochemical characterization and role in meat tenderization

Biography

Biography: Preeti Chanalia

Abstract

Proline iminopeptidase (PIPs) specifically cleaves N-terminal proline from peptides and helps in overcoming restriction of many aminopeptidases to cleave proline rich peptides/proteins. PIPs are used in food processing, meat tenderization as well as in treating collagen rich wastewater to generate bioactive collagen hydrolysates important for food and pharmaceutical industry. Microbial enzymes account for about 60% of total worldwide sale of enzymes. There is increasing demand of industrial enzymes with novel characteristics for different applications. Among microorganisms lactic acid bacteria have gained great attention because of their GRAS status (generally regarded as safe) and probiotic attributes. Investigation and development of highly potent probiotic consortium (comparable to commercially available products/Probiotics) suitable for functioning in adverse conditions is of great value as therapeutics as well as in various industries. The possibility of formation of mono- strains into multi-strain probiotics with potential of improved efficacy is the goal of this kind of studies. Methodology: Purification using successive column chromatographies, characterization, in silico studies of PIP from probiotic L. plantarum and its use in collagen degradation and meat tenderization by simple and effective treatment with enzyme/s was done. Findings: Membrane bound PIP from L. Plantarum was extracted, purified and characterized further. The effectiveness of PIP in purified form, membrane bound form and in combination with other enzymes to degrade collagen and meat tenderization marks its industrial importance. Conclusion & significance: Purified PIP as well as whole cells effectively hydrolyzed collagen. Whole cells, PIP alone and with other enzymes tenderized chicken meat efficiently. It is very significant for food, pharmaceutical industry and waste management. It also opens avenue for the formation of multi strain probiotics.

References:

  1. Dimpi Gandhi, Preeti Chanalia, Pooja Attri, Suman Dhanda. Dipeptidyl peptidase-II from probiotic Pediococcus acidilactici: Purification and functional characterization. International Journal of Biological Macromolecules. 2016, 93,919:932.
  2. Preeti Chanalia, Dimpi Gandhi, Raman Kumar, Pooja Attri, Suman Dhanda. Lactic acid production vis-à-vis biowaste management using lactic acid bacteria. World Applied Science Journal. 2016.(Accepted)    
  3. Pooja Attri, Drukshakshi Jodha, Dimpi Gandhi, Preeti Chanalia, Suman Dhanda. In vitro evaluation of Pediococcus acidilactici NCDC 252 for its probiotic attributes. International Journal of Dairy Technology. February 2015.  DOI: 10.1111/1471-0307.12194.
  4. Prabodh Chander Sharma, Ankit Jain, Mohammad Shahar Yar, Rakesh Pahwa, Jasbir Singh, Preeti Chanalia. Novel fluoroquinolone derivatives bearing N-thiomide linkage with 6-substituted-2-aminobenzothiazoles: synthesis and antibacterial evaluation. Arabian Journal of Chemistry.  November 2012.  DOI: 10.1016/j.arabjc.2012.11.002.
  5.  Preeti Chanalia, Dimpi Gandhi, Drukshakshi Jodha, Jasbir Singh. Applications of microbial proteases in pharmaceutical industry: an overview. Reviews in medical microbiology. 2011, 22: 96-101.